Abstract: The main research of this experiment is to add a newly developed Bacillus megaterus preparation (BM1259 preparation) to the diet and determine its effect on the performance of laying hens. 6 986 Roman hens of the same age, same batch, and similar production performance were selected and divided into 2 groups, which were kept in two chicken houses with the same structure and size. The basic diet and normal drinking water were used as the control group, and the daily ton was 400 g of BM1259 preparation was added to the diet as the test group. There were 3 504 chickens in the control group and 3 482 chickens in the test group. The results showed that: (1) The addition of BM1259 could increase the laying rate of laying hens, which was 3.48% higher than that of the control group (P<0.05). (2) The average egg weight of the experimental group was 0.10% lower than that of the control group, but the difference was Not significant (P>0.05).
Key words: Bacillus; production performance; laying hens
In recent years, microecological preparations have attracted much attention due to their natural, non-toxic side effects, no residue, and no pollution to the environment. The application range is very wide. At present, there are many reports on microecological preparations mainly based on Lactobacillus abroad, followed by Bacillus.
A newly developed Bacillus preparation was used in this test. Bacillus can promote the growth of beneficial intestinal flora and inhibit harmful bacteria, help to degrade some complex compounds in feed, improve feed utilization, promote body growth and improve animal performance. This trial hopes to provide reliable data for the application of the product in laying hen production by studying its effect on laying performance of laying hens.
1 Materials and methods
1.1 Test time and test location
The experiment was conducted at the breeding farm in Hai'an County, Jiangsu Province from March 4 to April 11, 2009. The pre-test period is 7 days and the trial period is 32 days.
1.2 Test micro-ecological preparations
Bacillus megater preparation (BM1259 preparation): Micro-organic live bacterial preparation, provided by the College of Animal Science and Technology of Yangzhou University.
1.3 Test Design
A total of 6 986 Romanian laying hens of 240 days old were selected, with an average weight of 1.8 kg. They were randomly divided into the experimental group and the control group, with 3 482 in the experimental group and 3 504 in the control group. There was no significant difference in egg production rate and average egg weight between the two groups before the test. The control group was fed a basal diet (Table 1). The test group added 400 g of Bacillus megaterium preparation per ton in the basal diet and stirred well.
Table 1 Basic diet composition and nutritional level%
| raw material | proportion |
| corn | 63 |
| Cardamom | 26 |
| Stone powder | 8 |
| Premix | 3 |
| Nutritional level | |
| Metabolic energy / (MJ / kg) | 11.5 |
| Crude protein | 16.5 |
| calcium | 3.1 |
| Total phosphorus | 0.65 |
Note: The composite premix of 3% laying hens is the Hualuo premix produced by Zhongmu Industrial Co., Ltd. Each kilogram of premixed diet provides: VA 200,000 to 330,000 IU, VE ≥ 380 IU, VB1 ≥ 65 mg, VB2 ≥ 180 mg, VB6 ≥ 90 mg, VB12 ≥ 0.4 mg, VD3 80,000 to 150,000 IU, VK3 60 to 100 mg , folic acid ≥ 19mg, niacin ≥ 690mg, calcium pantothenate ≥ 320mg, biotin ≥ 1.2mg, choline chloride ≥ 11 000mg, iron 2 600 ~ 5 000mg, copper 240 ~ 470mg, zinc 2 500 ~ 4 000mg, manganese 3 000 ~ 5 000mg, iodine 30 ~ 55mg, selenium 9 ~ 15mg, calcium 14% ~ 18%, total phosphorus ≥ 4.0%, salt 8% ~ 12%, water ≤ 10%.
1.4 Feeding Management
The two groups were housed and managed according to conventional production management procedures and immunization procedures. Two-component two chicken houses are housed in three-step cages, artificially fed, artificially cleaned, and nipple-type drinkers are free to drink. Feed dry powder and feed twice a day (4:30, 14:00). The two chicken houses are identical in size and structure, with a spacing of 4m and natural ventilation. The combination of natural light and artificial light photography, the light time during the laying period is 16h. The test chickens were observed daily for feeding, feces and mental status. Eggs were picked at 14:30 every day during the test and the house temperature was recorded at 10:00 every day.
1.5 Test indicators
According to the group, the egg production is counted daily and the production performance indexes such as egg production rate and egg weight are calculated.
1.6 Data Processing and Analysis
After preliminary analysis by Excel2003, the data obtained from the test were analyzed by SPSS10.0 statistical software. The t test was used to carry out the significance test. The results were expressed as “mean±standard errorâ€.
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